為了解山東省鴨坦布蘇病毒（duck tembusu virus，DTMUV）的流行與變異情況，對2018年下半年至2019年上半年泰安、濟寧和淄博等地送檢的臨床病鴨樣品進行了DTMUV病原學(xué)檢測，并對陽性病料進行病毒分離鑒定及分離株的全基因組序列分析。結(jié)果顯示：從102份樣品中，共檢測到8份DTMUV陽性，陽性率檢出率為7.8%，從陽性病料中分離到3株DTMUV分離株；3個分離株基因全長均為10 993 bp，彼此間的氨基酸同源性為99.2%~99.3%，與24個參考毒株的同源性為95.8%~99.9%，均屬于中國株I型；與參考毒株相比，分離株的核衣殼蛋白C、囊膜蛋白E和非結(jié)構(gòu)蛋白NS1的同源性相對較低，分別為86.1%~99.2%，95.0%~99.4%和92.3%~99.7%，同時存在數(shù)量不等的氨基酸突變位點，但關(guān)鍵的蛋白結(jié)構(gòu)和潛在的糖基化位點無差異。結(jié)果表明，DTMUV在山東部分地區(qū)仍有流行，但流行毒株基因結(jié)構(gòu)及致病特性與經(jīng)典毒株相比無明顯變化，但應(yīng)持續(xù)關(guān)注和深入研究。
Isolation and Identification of Shandong Strain of Duck Tembusu Virus
In order to identify the prevalence status and variation of duck Tembusu virus（DTMUV）in Shandong Province，the clinical samples delivered from Tai'an，Jining and Zibo during the second half of 2018 to the first half of 2019 were detected for DTMUV，and virus isolation and identification were carried out towards the positive samples，then the whole genome sequences of the isolates were analyzed. The results showed that 8 out of 102 samples were positive，with the detection rate of 7.8%，from which，3 strains of DTMUV were isolated；the full lengths of all the three strains were all 10 993 bp，the amino acid homology of they ranged from 99.2% to 99.3%，and the homology between the isolates and 24 reference strains ranged from 95.8% to 99.9%，all the isolates fell into Chinese strain I；compared to the reference strains，the homologies of nucleocapsid protein C，envelope protein E and non-structural protein NS1 of the isolates were lower，which were 86.1% to 99.2%，95% to 99.4% and 92.3% to 99.7%，respectively，and with different number of amino acid mutation sites，but major protein structures and potential glycosylation sites had no difference. It was concluded that DTMUV was still prevalent in some regions in Shandong Province，which should be further concerned and studied，although its genetic structure and pathogenicity remained unchanged compared to the classical strains.
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