為揭示黃芪防控豬繁殖與呼吸綜合征（PRRS）的物質(zhì)基礎(chǔ)及靶點(diǎn)信息，采用網(wǎng)絡(luò)藥理學(xué)方法，從中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫與分析平臺(tái)（TCMSP）篩選黃芪的潛在活性成分、作用靶點(diǎn)，同時(shí)從比較毒理基因組學(xué)數(shù)據(jù)庫（CTD）收集PRRS相關(guān)宿主基因，最后構(gòu)建“活性成分-關(guān)鍵靶點(diǎn)”網(wǎng)絡(luò)、蛋白互作網(wǎng)絡(luò)，并進(jìn)行GO功能和KEGG通路富集分析。結(jié)果顯示，從黃芪篩選出的17個(gè)潛在活性成分中，有14個(gè)潛在活性成分共計(jì)65個(gè)基因靶點(diǎn)與PRRS相關(guān)宿主基因交叉，其中槲皮素、山奈酚、刺芒柄花素、異鼠李素等潛在活性成分可能對(duì)治療PRRS有重要作用，TNF、IL8、TP53、IL6、IL10、IL1B、JUN等45個(gè)蛋白靶點(diǎn)間存在相互作用。此外，黃芪治療PRRS的作用機(jī)制可能涉及細(xì)胞外間隙和胞漿等組分，并可能與炎癥反應(yīng)、細(xì)胞凋亡、T細(xì)胞受體信號(hào)通路等生物過程相關(guān)。本研究為臨床應(yīng)用黃芪及其提取物治療PRRS奠定了理論基礎(chǔ)。
Analysis on the Material Basis and Drug Targets of Astragalus Membranaceus in Prevention and Control of PRRS Based on Network Pharmacology
In order to reveal the information concerning the material basis and drug targets of astragalus membranaceus acting on porcine reproductive and respiratory syndrome（PRRS），the potential active components and drug targets related to astragalus membranaceus were screened through traditional chinese medicine systems pharmacology database and analysis platform（TCMSP）by the network pharmacology. Meanwhile，the host genes related to PRRS were gathered from comparative toxicogenomics database（CTD）to construct the“active components-key targets”network and protein-protein interaction（PPI）network，and to analyze the GO functions and KEGG pathway enrichment. The results showed that 14 out of 17 potential active components including 65 gene targets crossed with the host genes related to PRRS，especially，quercetin，kaempferol，formononetin，isorhamnetin and other potential active components played an important role in fighting against PRRS，and 45 targets could interact with each other，including TNF，IL8，TP53，IL6，IL10，IL1B and JUN. In addition，the mechanism of astragalus membranaceus in fighting against PRRS might involve extracellular space and cytoplasmic components，and might be related to biological processes such as inflammatory response，apoptosis，and T cell receptor signaling pathway. Therefore，a theoretical foundation was laid for clinical application of astragalus membranaceus and its extract in fighting against PRRS.
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